pb tre cas9 vpr (Addgene inc)

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Addgene inc pb tre cas9 vpr
Pb Tre Cas9 Vpr, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 78 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pb+tre+dcas9+vpr/PB-TRE-dCas9-VPR+(Plasmid+%2363800)/bio_rxiv__64898__2026__01__30__700779-195-13-14
Average 95 stars, based on 78 article reviews

pb tre cas9 vpr - by Bioz Stars, 2026-09

95/100 stars

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Plasmid Preparation:

Article Title: A genome-wide genetic screen uncovers determinants of human pigmentation
Article Snippet: In the screen hit validation experiment, the Pb459-U6-sgRNA-EF1a-PuroR vector (Addgene # 195507) used for sgRNA expression in the Cas9-MNT-1 cells was designed in two steps. .. First, the lentiviral backbone of the pMCB320 vector was replaced with the piggyBac backbone from 135 the PB-TRE-dCas9-VPR (Addgene, 63800) vector using EcoRI (NEB) and XbaI (NEB) restriction enzymes. .. Next, PuroR-T2A-mCherry was replaced with the PuroR cassette using NheI (NEB) and EcoRI (NEB).

Article Title: Elucidating the genetic mechanisms governing cytosine base editing outcomes through CRISPRi screens
Article Snippet: .. The backbone of the rA1-SaBE4(ΔUGI) PiggyBac transposon plasmid, pSG236, was adopted from PB-TRE-dCas9-VPR (Addgene, #63800). rA1-SaBE4( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\Delta$$\end{document} Δ UGI) was assembled into the PiggyBac backbone with USER cloning to produce pSG236. .. The backbone of both reporter vectors was adopted from pU6-sgRNA EF1α-puro-T2A-BFP (Addgene, #60955).

Article Title: CRISPR-based screening identifies the role of KRAB-containing transcription factors ZIM3 and ZNF394 in human major zygotic genome activation.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bowtie2 v2.5.4 Langmead et al.59 https://bowtie-bio.sourceforge.net/bowtie2/ index.shtml MAC2 v2.1.4 Zhang et al.60 https://github.com/jdavisturak/MACS2-2.1.1.20160309 Deeptools v3.3.0 Ramı́rez et al.61 https://test-argparse-readoc.readthedocs.io/ en/latest/content/installation.html CHIPseeker v1.30.3 Wang et al.62 https://bioconductor.org/packages/release/ bioc/html/ChIPseeker.html DiffBind v3.4.11 Cancer Research UK’s Cambridge Research Institute https://bioconductor.org/packages/release/ bioc/html/DiffBind.html MEME-suite v5.5.7 Timothy et al.63 https://meme-suite.org Graphpad Prism v9.0 Dotmatics https://www.graphpad.com/scientific-software/ prism/www.graphpad.com/scientific-software/ prism/ Other GTRD database Yevshin et al.49 http://gtrd.biouml.org DAVID database for GO and KEGG analysis Sherman et al.64 https://davidbioinformatics.nih.gov/ STRING database for PPI analysis Szklarczyk et al.65 N/A hORFeome Broad Institute N/A Cell Reports 44, 116015, August 26, 2025 23 .. For plasmid PB-idCas9-VPR-NeoR, the backbone vector was originated from PB-TRE-dCas9-VPR (addgene#63800), while the HygR sequence was replaced by NeoR sequence. .. For plasmid PB-DUXA-pMaxGFP-NLS-PGK-HygR, the regulatory sequence started from − 2 kb to transcription start site (TSS) of human DUXA gene was synthesized by Genscript.

Article Title: Elucidating the genetic mechanisms governing cytosine base editing outcomes through CRISPRi screens.
Article Snippet: .. The backbone of the rA1-SaBE4(ΔUGI) PiggyBac transposon plasmid, pSG236, was adopted from PB-TRE-dCas9-VPR (Addgene, #63800). rA1-SaBE4(ΔUGI) was assembled into the PiggyBac backbone with USER cloning to produce pSG236. .. The backbone of both reporter vectors was adopted from pU6sgRNA EF1α-puro-T2A-BFP (Addgene, #60955).

Cloning:

Construct:

Article Title: TKTL1: a new candidate gene in non-obstructive azoospermia
Article Snippet: .. PB-TRE-dCas9-VPR (Addgene number 63,800) (Addgene, Watertown, MA, USA) was purchased by the Max Planck Institute for Molecular Genetics in Berlin (Department Herrmann), which has the right to use the construct. ..

Sequencing:

Transfection:

Article Title: Transcriptional Dosage of Oncogenic KRAS Drives Lung Adenocarcinoma Cell States, Progression and Metastasis
Article Snippet: .. KPE-luc transfection was conducted using Fugene HD transfection reagent (Promega) with doxycycline-inducible plasmids: PB-TRE-dCas9-VPR (Addgene #63800) and PB-TRE-dCas9-KRAB-MeCP2 (Addgene #122267). ..

other:

Article Title: Programmable Nucleic Acid Sensing in Human Cells Using Circularizable ssDNA
Article Snippet: To introduce custom sequences, the 3′ UTR of the original construct was replaced with a synthetic sequence generated by annealing two complementary oligonucleotides (IDT).

Review